multitag pyrosequencing  (Pyrosequencing Inc)

Journal: Frontiers in Medicine

Article Title: Hepatic encephalopathy treatment after transjugular intrahepatic portosystemic shunt: a new perspective on the gut microbiota

doi: 10.3389/fmed.2025.1423780

Figure Lengend Snippet: Role of gut microbiota in diagnosing and predicting HE.

Article Snippet: Bajaj et al. ( ) , N = 60 patients with cirrhotic ( N = 36HE vs. N = 24 non-HE) and controls ( N = 17) , Sigmoid biopsies and Fecal samples , Multitag pyrosequencing , Between patients with/without HE patients, there was no difference in stool microbiota, but the mucosal microbiome was different with lower Roseburia and higher Enterococcus, Veillonella, Megasphaera, and Burkholderia abundance in HE. , The mucosal microbiota of patients with cirrhosis, especially those with HE, lack potentially beneficial autochthonous genera and have overgrowth of potentially pathogenic genera, which are associated with poor cognition and inflammation..

Techniques: Control, Bacteria, Biomarker Discovery, Sequencing, Functional Assay

Journal: Microorganisms

Article Title: Human Gut Microbiome and Liver Diseases: From Correlation to Causation

doi: 10.3390/microorganisms9051017

Figure Lengend Snippet: Important studies of human microbiome and liver diseases.

Article Snippet: Mutlu et al., 2012, Am J Physiol Gastrointest Liver Physiol [ ] , Human sigmoid mucosa biopsy , ALD , Multitag-pyrosequencing , The first study showing the association between alcohol consumption and microbiome using non-culture method in human. Lower abundances of Bacteroidetes and higher ones of Proteobacteria was found in alcoholics..

Techniques: Real-time Polymerase Chain Reaction, Sequencing, Shotgun Sequencing, Biomarker Discovery